Journal: Journal of acquired immune deficiency syndromes (1999)

Article Title: 30-day readmission rates in an HIV-infected cohort from Rio de Janeiro, Brazil

doi: 10.1097/QAI.0000000000001352

Figure Lengend Snippet: Socio-demographic and clinical characteristics and unadjusted hazard ratios (95% confidence interval) for patients who hospitalized stratified by readmission, INI cohort.

Article Snippet: Compared to patients not readmitted, those who were readmitted were more likely to have recent (≤30 days) HIV infection diagnosis (14.9% vs. 8.5% among non-readmitted, Chi-squared p-value=0.019) and lower CD4 counts (median 118 cells/mm 3 vs. 191 cells/mm 3 among non-readmitted, Wilcoxon Rank-Sum test p-value<0.0001).

Techniques: Biomarker Discovery

Journal: Scientific Reports

Article Title: Stability, enrichment, and quantification of total and HPV16-specific IgG present in first-void urine

doi: 10.1038/s41598-024-65257-0

Figure Lengend Snippet: Study design. To assess storage and stability of IgG in FVU, eleven women provided a FVU sample collected without UCM. To 10 ml of the FVU sample, UCM was added in a 1:2 UCM:FVU ratio. The samples were aliquoted in 4 ml samples and stored at RT for different durations whereafter they were stored at – 80 °C. All samples were processed using Amicon filtration. To evaluate different IgG enrichment methods, eleven women provided both blood samples and two pooled FVU samples. From the blood samples, serum was extracted, while the pooled FVU samples were aliquoted in 4 ml samples and processed using different protocols. The different IgG enrichment protocols were: Amicon filtration (AM), AM followed by melon gel purification (AM-MG), protein G magnetic bead purification using glycine elution (PGMB-G) and citric acid elution (PGMB-CA). All samples were analyzed for total human IgG using the HTRF and AlphaLISA technology from PerkinElmer, as well as the BioPlex isotyping assay from Bio-Rad. Additionally, HPV16-IgG concentrations were measured using the HPV16 DELFIA.

Article Snippet: Significant correlation coefficients were found for AlphaLISA vs. BioRad (0.67 (95% CI 0.02–0.96)) and BioRad vs. HTRF (0.72 (95% CI 0.08–0.99)) for AM enriched samples.

Techniques: Filtration, Gel Purification, Purification

Journal: Scientific Reports

Article Title: Stability, enrichment, and quantification of total and HPV16-specific IgG present in first-void urine

doi: 10.1038/s41598-024-65257-0

Figure Lengend Snippet: Total IgG and HPV16-IgG results for different storage conditions of FVU. ( A ) Boxplots of total human IgG concentrations for 11 samples stored under different conditions using the BioPlex, the HTRF assay and the AlphaLISA. ( B ) Boxplots of HPV16-IgG concentrations. Significance levels are represented in the figure by an asterisk (*p < 0.05, **p < 0.01, ***p < 0.001). Outliers in all figures represent the same ID.

Article Snippet: Significant correlation coefficients were found for AlphaLISA vs. BioRad (0.67 (95% CI 0.02–0.96)) and BioRad vs. HTRF (0.72 (95% CI 0.08–0.99)) for AM enriched samples.

Techniques: HTRF Assay

Journal: Scientific Reports

Article Title: Stability, enrichment, and quantification of total and HPV16-specific IgG present in first-void urine

doi: 10.1038/s41598-024-65257-0

Figure Lengend Snippet: Spearman correlation plots for all stability data combined ( A ) Correlation plot between the AlphaLISA and the BioRad assay. ( B ) Correlation plot between the BioRad assay and the HTRF assay. ( C ) Correlation plot between the AlphaLISA and the HTRF assay. Spearman’s rank correlation coefficient presented in the figure are for all conditions combined. Different symbols represent IDs and different colors represent buffer conditions in the correlation plots. Outliers in all figures represent the same ID.

Article Snippet: Significant correlation coefficients were found for AlphaLISA vs. BioRad (0.67 (95% CI 0.02–0.96)) and BioRad vs. HTRF (0.72 (95% CI 0.08–0.99)) for AM enriched samples.

Techniques: HTRF Assay

Journal: Scientific Reports

Article Title: Stability, enrichment, and quantification of total and HPV16-specific IgG present in first-void urine

doi: 10.1038/s41598-024-65257-0

Figure Lengend Snippet: Total IgG and HPV16-IgG results in FVU of the IgG enrichment cohort. ( A ) Boxplots of total human IgG concentrations after different enrichment protocols for 11 FVU samples using the BioPlex, the HTRF assay and the AlphaLISA. ( B ) Boxplots of HPV16-IgG concentrations after different enrichment protocols for 11 FVU samples. Significance levels are represented in the figure by an asterisk (*p < 0.05, **p < 0.01, ***p < 0.001). Different symbols represent different IDs. Results for TRF based methods (HTRF and DELFIA) are invalid for PGMB enrichment methods due to non-specific binding.

Article Snippet: Significant correlation coefficients were found for AlphaLISA vs. BioRad (0.67 (95% CI 0.02–0.96)) and BioRad vs. HTRF (0.72 (95% CI 0.08–0.99)) for AM enriched samples.

Techniques: HTRF Assay, Binding Assay

Journal: Scientific Reports

Article Title: Stability, enrichment, and quantification of total and HPV16-specific IgG present in first-void urine

doi: 10.1038/s41598-024-65257-0

Figure Lengend Snippet: Correlation coefficients for the different total human IgG quantification methods for different FVU IgG enrichment methods and serum.

Article Snippet: Significant correlation coefficients were found for AlphaLISA vs. BioRad (0.67 (95% CI 0.02–0.96)) and BioRad vs. HTRF (0.72 (95% CI 0.08–0.99)) for AM enriched samples.

Techniques: